Journal: Skeletal Muscle

Article Title: GsMTx4-blocked PIEZO1 channel promotes myogenic differentiation and alleviates myofiber damage in Duchenne muscular dystrophy

doi: 10.1186/s13395-025-00383-5

Figure Lengend Snippet: GsMTx4-blocked PIEZO1 channel ameliorates muscular dystrophy in mdx mice. ( A ) Representative Western blot of dystrophin in muscle tissue of WT and mdx mice. ( B ) Flow diagram of the treatment in mdx mice, the red markings indicate the time points for drug injections. ( C ) Representative H&E images show myofibers treated with GsMTx4 for 30 days. ( D-F ) The cross-sectional area of myofibers and grip strength were increased, and the proportion of centrally nucleated fibers was decreased after treatment with GsMTx4 ( n = 5; mean ± SD; Student’s t-test). ( G ) Representative H&E images show myofibers treated with Yoda1 for 30 days. ( H-J ) The cross-sectional area of myofibers and grip strength were decreased after treatment with Yoda1 ( n = 5; mean ± SD; Student’s t-test). Statistical significance was set at P < 0.05. * P < 0.05. Scale bar, 50 μm

Article Snippet: Overnight incubation at 4 °C followed with primary antibodies against PIEZO1 (1:1000, Proteintech, #15939-1-AP), Dystrophin (1:500, Santa Cruz, #sc-365954), and p-CaMKII (1:200, Santa Cruz, #sc-32289).

Techniques: Western Blot